Optimum working dilutions should really be determined experimentally by the investigator. Proposed beginning dilutions are the following: WB 1:5000, IP: 1:200, IF: 1:1000.
The antibody was affinity-purified from mouse ascites by affinity-chromatography using particular immunogen.
Immunofluorescence staining (1:1,000) of VSV-G combination protein in 293 cells with red and counterstained with DAPI.
IP (1:200) - WB (1:5,000) evaluation of VSV-G Tag fusion protein phrase in 293 cells. Untransfected 293 mobile lysate (lane A), transfected 293 cell lysate with VSV-G label protein (lane B); IP untransfected 293 mobile lysate with Anti VSV-G draw mAb (lane C); IP Vesicular stomatitis virus glycoprotein tag antibody 293 cell lysate with regular Mouse IgG (lane D) or with Anti VSV-G tag mAb (lane E).
Western mark evaluation of 1ug VSV-G combination protein with Anti-VSV-G mouse monoclonal antibody in 1:5,000 (lane A) and 1:10,000 (lane B) dilutions.
Water in PBS, pH 7.4, comprising 0.02% sodium azide as preservative and 50% Glycerol.
Stable for twelve months at -20°D from date of shipment. For optimum recovery of solution, centrifuge the initial vial after thawing and prior to removing the cap. Aliquot to avoid recurring cold and thawing.
The item stated herein is for research use just and isn't intended for use within human or medical diagnosis. Proposed applications of our products are not suggestions to utilize our items in violation of any patent or as a license. We can not lead to patent infringements and other violations that will occur with the usage of that product.
The fusiogenic package G glycoprotein of the vesicular stomatitis disease (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone being an successful car for gene transfer. The VSV-G epitope tag is frequently engineered onto the N- or C- terminus of a protein of fascination so the tagged protein can be examined and visualized applying immunochemical methods.