Optimum functioning dilutions ought to be decided experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:5000.The antibody was affinity-purified from mouse ascites by affinity-chromatography using unique Major capsid protein antibody.
American blot examination of 0.5ug T7 Draw fusion protein with Anti-T7 Tag monoclonal antibody in 1:1,000 (lane A), 1:2,000 (lane B), 1:5,000 (lane C) dilutions.Liquid in PBS, pH 7.4, comprising 0.02% sodium azide as preservative and 50% Glycerol.
Secure for 12 months at -20°D from day of shipment. For optimum healing of product, centrifuge the first vial following thawing and just before eliminating the cap. Aliquot to prevent repeated freezing and thawing.
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T7 label is definitely an epitope label made up of an 11-residue peptide secured from the leader series of the T7 bacteriophage gene10, which encodes a T7 major capsid protein. The T7 tag is commonly manufactured onto the N- or C- terminus of a protein of interest so your branded protein could be reviewed and visualized using immunochemical methods.
The T7 label has been applied extensively as a general epitope draw in several appearance vectors including the pET program that is based on T7 RNA polymerase phrase systems.T7 draw; 10; Major capsid protein 10A; Important capsid protein; Modest capsid protein 10B; T7 epitope.